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Molecular Devices

MycoAlert Mycoplasma Detection assays

Protocol File MycoAlert (6.4).spr     Download
The MycoAlertâ„¢ Assay and MycoAlert PLUS Assay from Lonza provide a rapid and convenient way to detect viable mycoplasma in cell cultures using a luminescence microplate reader. The assays take advantage of mycoplasma specific enzymes found in major mycoplasma species to screen for contamination.
Showcases Data Analysis
Read Mode Luminescence
SoftMax Pro Version 6.4
Primary Instrument Used SpectraMax i3x
Suitability Any

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Added By Cathy Olsen Added On 2016-02-19 00:26:31 UTC
Last Updated By Cathy Olsen Last Updated On 2016-02-19 00:26:31 UTC
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Can you provide the protocol file in an earlier version of SoftMax Pro? We are using version 6.3 Timothy Johns
2016-03-15 20:16:37 UTC

Hello Timothy, I just posted a version of this protocol for SoftMax Pro 6.1. Thanks for your feedback. Cathy Olsen
2016-03-15 21:28:02 UTC

I am testing out this protocol on our Paradigm plate reader with a TUNE cartridge SotMaxPro 6.4.1, and I have a question regarding the read height adjustment.

The protocol initally loads with a 10mm read height setting for both reading A and B, but the volumes change between the two readings so should read height be optimised for each reading instead? My plate reader suggest around 8mm for reading A and around 11-12mm for reading B - but the latter seems to be very far from the top, so would this increase risk of spill-over luminescence from nearby wells?
Freja Venning
2017-03-09 09:41:32 UTC

Hi Freja, crosstalk from adjacent wells is always a concern we think about, but the read height optimization is still a useful tool to maximize signal. It would be worth a pilot study, though, where you can optimize in an isolated well, check your RLUs, then add some bright positive controls to the adjacent wells and recheck. If your optimized heights aren't too susceptible to the crosstalk, you should be good. If you are getting increased signal from adjacent well crosstalk, I would manually move the read height down in increments. I hope this helps! Bryan Conn
2017-03-16 17:14:54 UTC

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